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. 2016 Jan 6;10(5):735–750. doi: 10.1016/j.molonc.2015.12.012

Figure 5.

Figure 5

Selective HDAC6 inhibitors down‐regulate PD‐L1 expression in vitro. (A) WM164, WM983A and WM793 human melanoma cell lines were incubated with the HDAC6 inhibitor Tubastatin A (3 μM) for 24 h, followed for IL‐6 stimulation (30 ng/mL). Then, the cells were lyzed and immunoblotted using the specific antibodies listed in figure. (B) WM164 cells were treated with Tubastatin A (3 μM), with or without stimulation of IL‐6 and then the expression of PD‐L1 was analyzed by flow cytometry.