Figure 5.
Selective HDAC6 inhibitors down‐regulate PD‐L1 expression in vitro. (A) WM164, WM983A and WM793 human melanoma cell lines were incubated with the HDAC6 inhibitor Tubastatin A (3 μM) for 24 h, followed for IL‐6 stimulation (30 ng/mL). Then, the cells were lyzed and immunoblotted using the specific antibodies listed in figure. (B) WM164 cells were treated with Tubastatin A (3 μM), with or without stimulation of IL‐6 and then the expression of PD‐L1 was analyzed by flow cytometry.