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. 2016 Jan 6;10(5):735–750. doi: 10.1016/j.molonc.2015.12.012

Figure 7.

Figure 7

Manipulation of HDAC6 modulates the expression of membrane co‐stimulatory molecules and other regulatory proteins. NT and HDAC6KD WM164 human melanoma cells were stimulated with IFNγ (100 ng/ml) or left untreated. Next, the presence of PD‐L2, TRAIL‐R1, TRAIL‐R2, B7‐H3 and B7‐H4 were measured by western blot (A) and flow cytometry (B). (C) Cells from melanoma patient's biopsies were treated or untreated with Nexturastat (3 μM and 6 μM) and then the presence of HDAC6, PD‐L2, B7‐H3, B7‐H4, TRAIL‐R1, TRAIL‐R2, Galectin 9 and GAPDH was evaluated by immunoblot.