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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Mol Neurobiol. 2015 Nov 17;53(9):6377–6387. doi: 10.1007/s12035-015-9536-0

Figure 1. PDGF-BB promotes neuronal differentiation of rat primary NPCs.

Figure 1

Representative blots (a) and RT-PCR (b) showing the protein as well as gene expression level of Nestin, a NPC maker in control group and the PDGF-BB treated group after one, three, and five days culture; (c) Effect of PDGF-BB on the cell survival of rat primary NPCs using the MTT assay. (d) Representative immunofluorescence images showing the expression of neuronal maker β-III-Tubulin (Red), astrocyte maker GFAP (Green) and nuclei DAPI staining (Blue) in NPCs after 5days culture in control or PDGF-BB treated group. Scale bar: 20 μm; (e) Representative blots and quantification of the blot density (f)representative blots showed the expression pattern of β-Tubulin III and GFAP in NPCs cultured for 1day, 3 days and 5 days with PDGF-BB exposure. All the data in these figures are presented as mean±S.D. of at least three individual experiments. *P<0.05 versus control group, **P<0.01 versus control group.