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. 2016 May 3;39(5):426–438. doi: 10.14348/molcells.2016.0094

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Fig. 1. — AVR-Pii preferentially interacts with Os-NADP-ME2-3. A-D, Interaction of AVR-Pii with Os-NADP-ME2-3 in Yeast-two hybrid (Y2H) assays. (A) Positive interactions were detected by five different tests, followed by the appearance of blue color in the X-gal assay and cell growth on synthetic complete-leucine-tryptophan-histidine (SC-LTH) including 55 mM 3-amino-1,2,4-triazole (3-AT), SC-leucine-tryptophan (SC-LT) containing 0.2% [w/v] 5-fluoroorotic acid (SC-FOA), SC-uracil (SC-URA) and SC-LT medias. Controls used were as follows: strong (pEXP32/Krev1 + pEXP22/RalGDS-wt); weak (pEXP32/Krev1 + pEXP22/RalGDS-m1); and absent (pEXP32/Krev1 + pEXP22/RalGDS-m2). (B) Thirteen different sizes of interacting Os-NADP-ME2-3 cDNAs with AVR-Pii were identified, and their detection frequencies are indicated. (C) AVR-Pii showed interaction specificity with the Os-NADP-ME2-1 and Os-NADP-ME2-3 alternative splice forms. (D) No interaction was detected between Os-NADP-ME2-3 and AVR-Pia, AVR-Pizt and AVR-Pikm.