Fig. 6.
Double reciprocal plot for the inhibition of Os-NADP-ME2-3 by AVR-Pii and AVR-Pii-MT. The activity of Os-NADP-ME2-3 alone and in the presence of AVR-Pii or AVR-Pii-MT was determined using NADP+ as the variable substrate at 3.5 mM L-Malate (A) and (C) and using L-Malate as the variable substrate at 0.3 mM NADP+ (B) and (D). The data indicates AVR-Pii competitively inhibits Os-NADP-ME2-3, whereas a lack of inhibition was found with AVR-Pii-MT. The averages are from three independent repeats.