Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 18;7:753. doi: 10.3389/fmicb.2016.00753

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Wang, Wang, Zhang, Liu, Luo, Li, Cao, Liu, Xu and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Real-time detection of a single target in an ET-MCDA. Analytical sensitivity of Salmonella-ET-MCDA (A) and Shigella-ET-MCDA (B) for Salmonella and Shigella detection was analyzed by real-time format, and signals 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, and 13 represent DNA levels of 25 ng, 2.5 ng, 250 pg, 25 pg, 2.5 pg, 250 fg, 25 fg, 12.5 fg, 6.25 fg, 3.125 fg, 1.56 fg, and 0.78 fg per tube and negative control. The LoD of Salmonella-ET-MCDA approach was 6.25 fg per tube, and the Shigella-ET-MCDA for 3.125 fg per tube. Analytical sensitivity of Salmonella-ET-MCDA (C) and Shigella-ET-MCDA (D) for Salmonella and Shigella detection was also analyzed by 2% agarose gel electrophoresis, and the positive reactions were observed as a ladder-like pattern on 2% agarose gel electrophoresis analysis. Lane 1, DL 100-bp DNA marker.