Table 1.
Primers namea | Sequences and modifications | Length | Genesb |
---|---|---|---|
Sal-F1 | 5′-CCCCGCATTGTTGATTGC-3′ | 18 nt | |
Sal-F2 | 5′-CGCTACGTTTTGCTTCACG-3′ | 19 nt | |
Sal-CP1 | 5′-ATGATATTCCGCCCCATATTATCCG-GATTAGTGCCGGTTTTATCGTG-3′ | 47 mer | |
Sal-E-CP1 | 5′-Cy5-TGCAATG-ATGATAT(BHQ2)TCCGCCCCATATTATCCGGATTAGTGCCGGTTTTATCGTG-3′ | 54 mer | |
Sal-CP2 | 5′-ATGGGAACTCTGCCGGGATT-GAAGAGTACGCTTAAAACCACC-3′ | 42 mer | |
Sal-C1 | 5′-ATGATATTCCGCCCCATATTATCCG-3′ | 25 nt | invA |
Sal-C2 | 5′-ATGGGAACTCTGCCGGGATT-3′ | 20 nt | |
Sal-D1 | 5′-TATCGCCATTTACGCGGGT-3′ | 19 nt | |
Sal-D2 | 5′-GTTGTTCAACAGCTGCGTC-3′ | 19 nt | |
Sal-R1 | 5′-CCCACTGCCGGTTTTTGTT-3′ | 19 nt | |
Sal-R2 | 5′-GGTTGTTACGGCTATTTTGAC-3′ | 21 nt | |
Shi-F1 | 5′-GAAGAGCACGCCAACACC-3′ | 18 nt | |
Shi-F2 | 5′-TGATGGACCAGGAGGGTT-3′ | 18 nt | |
Shi-CP1 | 5′-CGACCTGTTCACGGAATCCGG-CTTGACCGCCTTTCCGATAC-3′ | 41 mer | |
Shi-E-CP1 | 5′-Hex-TGCAATG-CGACCT(BHQ1)GTTCACGGAATCCGGCTTGACCGCCTTTCCGATAC-3′ | 48 mer | |
Shi-CP2 | 5′-TCGACAGCAGTCTTTCGCTGTT-CCGGAGATTGTTCCATGTGA-3′ | 42 mer | |
Shi-C1 | 5′-CGACCTGTTCACGGAATCCGG-3′ | 21 nt | ipaH |
Shi-C2 | 5′-TCGACAGCAGTCTTTCGCTGTT-3′ | 22 nt | |
Shi-D1 | 5′-GGTATTGCGTGCAGAGAC-3′ | 18 nt | |
Shi-D2 | 5′-GTTTTTCCAGCCATGCAG-3′ | 18 nt | |
Shi-R1 | 5′-TGATGCCACTGAGAGCTGTGA-3′ | 21 nt | |
Shi-R2 | 5′-TCAGTGCCTCTGCGGAGCT-3′ | 19 nt |
Sal, salmonella; Shi, shigella.
mer, monomeric unit; nt, nucleotide.