Table 1.
Susceptibility of B. cenocepacia K56-2 to P. aeruginosa-derived small molecules.
| Medium | Spent medium (%)a | KCN (mM)b | PYO (μg ml−1)b | PCA (μg ml−1)b | RLs (μg ml−1)b |
|---|---|---|---|---|---|
| LB | 50 | 0.8 | >300 | >60 | ≥600 |
| LB1∕4 | >50 | 1.6 | >300 | >60 | N.D. |
| LB1∕10 | 50 | 3.2 | >300 | >60 | N.D. |
| BHI | >50 | 0.4 | >300 | 7.5 | N.D. |
| BHI1∕4 | >50 | 1.6 | >300 | >60 | N.D. |
| BHI1∕10 | 12.5 | 1.6 | >300 | >60 | N.D. |
| M9Glc | 12.5 | N.D. | N.D. | N.D. | N.D. |
| M9CAA | 12.5 | N.D. | N.D. | N.D. | N.D. |
| SCFM | 25 | >25 | >300 | >60 | N.D. |
MIC values after 24 h of growth in 96-well format using B. cenocepacia K56-2 are reported.
a
Spent medium was extracted from 24-h planktonic cultures of WT P. aeruginosa PA14 grown as described in the Materials and Methods section. The antimicrobial activity of each spent medium was determined in the same growth medium for which supernatants were extracted.
b
The MIC of purified compounds were determined in LB medium.
N.D., Not determined; KCN, potassium cyanide; PYO, pyocyanin; PCA, phenazine-1-carboxylic acid; RLs, di-rhamnolipids.