Figure 5.

Analysis of peptidoglycan-hydrolyzing activities of phage vB_BpuM_BpSp. The purified phage vB_BpuM_BpSp was mixed with loading buffer and boiled at 100°C for 10 min before separating them by SDS-PAGE. The purified Gp255 was mixed with loading buffer. After that, the unboiled (indicated as “Gp255”) and boiled (indicated as “Boiled Gp255”) proteins were separated by SDS-PAGE. The sterilized crude cell extracts of GR8 was added into the gel for lytic activity assay. The lane loaded with 1 × Loading buffer were used as control.