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. 2016 May 18;7:735. doi: 10.3389/fmicb.2016.00735

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Copyright © 2016 Ferreira and Garcia-Pichel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Absorption spectra of lipid-soluble pigment extracts of UV-A induced cells of N. punctiforme wild-type (WT) and its derived mutants SCY 59, ΔscyD, ΔscyE, and ΔscyF. The characteristic peak at 384 nm indicates the presence of scytonemin. (B) Scytonemin to chlorophyll a ratios in extracts of WT and mutant strains after 6 days of UV-A induction, as quantified by HPLC (Supplementary Figure S3). The asterisk mark (*) indicates that the scytonemin/chlorophyll a ratio in ΔscyF is significantly different than the WT (P < 0.05). Error bars correspond to standard deviations from five experimental replicates.