Table 2.

Effect of SARA induction on relative abundances of bacterial genera in the solid fraction using qPCR.¹

	Non-SARA		SARA		P-value2
	day 1	day 6	day 1	day 6	SG	Day	SG × Day
A. lipolytica	0.0024	0.0023	0.0008	0.0053	0.76	0.02	0.02
B. proteoclasticus	0.29	0.19	0.47	0.63	0.07	0.70	0.13
E. ruminantium	0.07	0.03	0.16	0.23	0.01	0.53	0.06
F. succinogenes3	0.0053	0.0010	0.0087	0.0076	0.20	0.08	0.13
M. elsdenii	8.1 × 10-4	7.2 × 10-4	4.3 × 10-4	1.0 × 10-3	0.94	0.41	0.27
P. bryantii3	3.1 × 10-4	2.1 × 10-4	7.6 × 10-4	3.4 × 10-3	0.09	0.22	0.04
S. ruminantium	0.08	0.05	0.04	0.05	0.59	0.47	0.14
S. amylolytica3	6.5 × 10-4	2.8 × 10-4	3.6 × 10-4	3.9 × 10-3	0.24	0.09	<0.01
S. bovis3	1.6 × 10-4	2.3 × 10-4	4.5 × 10-4	1.2 × 10-3	0.17	0.10	0.41
S. dextrinosolvens3	3.3 × 10-5	4.8 × 10-5	9.5 × 10-5	2.8 × 10-4	0.18	0.10	0.42
16S rRNA copy number4	4.6 × 106	4.9 × 106	4.2 × 106	4.6 × 106	0.75	0.39	0.97

¹Non-SARA = cows (n = 7) in which ruminal pH was not <5.6 for 3 h on day 5. SARA = cows (n = 5) in which ruminal pH was <5.6 for 3 h on day 5. ²SG = SARA grouping of cows based on ruminal pH as Non-SARA or SARA. ³Data were logit transformed to ensure normality of residuals. ⁴16S copy number/ng DNA.