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Expression analysis of PvRxLR28 in transgenic N. benthamiana. (A) RT-PCR analysis of PvRxLR28 expression in transgenic lines of T2 generation, and EF1-α was used as a control. (B) Western blot analysis of total proteins from transgenic N. benthamiana lines using monoclonal antibody against flag. The leaves for RT-PCR and western blot were collected at 2d post spraying of 17-β- estradiol (100 μM) and Silwet L-77 (0.01%).
