Table 1.
PCR primers, condition, and PRC-RELP patterns of different alleles of CTSB, CTSL, CTSS, CTSK, GHRH and LEPR genes in Ukrainian Large White pigs
| Gene | Primer sequences (5′ → 3′) | PCR conditions | Genotyping method | ||
|---|---|---|---|---|---|
| Length (bp) | Tm (°C) | MgCl2 (mM) | |||
| CTSB | F: GTGGCCGGGTGGGTTTTA R: TCCTCCTGGTGCTGCTAATTCTGAC |
139 | 55 | 2.0 | PCR–RFLP (MspI) |
| CTSL | F: TCACTGCCGTGAAGAATCAG R: GCAGAGCTGTAATGGCAAGA |
380 | 64 | 2.5 | PCR–RFLP (TaqI) |
| CTSS | F: AGAGAGCCAGAGGTTGCTCA R: GCAGGCAGAGCAAGCTAAA |
280 | 58 | 1.5 | PCR–RFLP (BseNI) |
| CTSK | F: TTGGGCGATATGGTGAGTTGAG R: CATAAGAAAGGAACCAAGGCAAACA Probe-G: VIC-CAGCTCCTGGTCTATC-NFQ Probe-A: FAM-TCAGCTCCTAGTCTATC-NFQ |
66 | 60 | 3.0 | Allelic discrimination |
| GHRH | F: GTAAGGATGC(C/T)(A/G)CTCTGGGT R: TGCCTGCTCATGATGTCCTGGA |
455 | 63 | 1.5 | PCR–RFLP(AluI): |
| LEPR | F: CAGAGGACCTGAATTTTGGAG R: CATAAAAATCAGAAATACCTTCCAG |
94 | 60 | 3.0 | HRM |