Table 1.
In vitro characterization of studied Ga-68 labelled siderophores. Log P, protein binding (expressed as % of protein bound activity of the total activity used) and stability (in human serum and 6 mM DTPA) of [68Ga]TAFC, [68Ga]FOXE, [68Ga]FCHA and [68Ga]FOXB
| [68Ga]siderophore | Log P (mean ± SD, n = 6) | Incubation time (min) | Protein binding (%) (mean, n = 2) | Stability in human serum (%) (n = 1) | Stability in DTPA solution (%) (n = 1) |
|---|---|---|---|---|---|
| [68Ga]TAFC | −2.59 ± 0.15 | 30 | 0.47 | 99.9 | 85.0 |
| 60 | 0.76 | 99.9 | 84.7 | ||
| 120 | 1.21 | 99.9 | 81.8 | ||
| [68Ga]FOXE | −1.65 ± 0.03 | 30 | 0.27 | 99.9 | 94.3 |
| 60 | 0.24 | 99.9 | 93.8 | ||
| 120 | 0.53 | 99.9 | 93.2 | ||
| [68Ga]FCHA | −3.24 ± 0.07 | 30 | 5.12 | 97.4 | 97.7 |
| 60 | 6.98 | 97.8 | 95.7 | ||
| 120 | 4.21 | 98.5 | 92.3 | ||
| [68Ga]FOXB | −3.56 ± 0.17 | 30 | 7.67 | 74.1 | 60.1 |
| 60 | 10.29 | 72.0 | 54.5 | ||
| 120 | 10.83 | 75.4 | 52.9 |