Skip to main content
. 2016 May 18;6:26119. doi: 10.1038/srep26119

Figure 3. Insulin signaling was impaired in the hippocampus of 26 weeks ApoE4xAPP mice.

Figure 3

(A–C) Immunoblots of hippocampal slices from APP, ApoE3xAPP (E3XAPP) and ApoE4xAPP (E4XAPP) mice were treated with or without 1 μM of insulin. Each blot is a representative of five independent experiments. Blot images were cropped for comparison and all relevant gels have been run under similar experimental conditions. Cropping area is indicated by black line surrounding the border of the blot figures. Densitometry analysis was performed using the NIH ImageJ software. Results were expressed as a fold change in protein expression as compared to the non-treated slices within the same mouse line. Error bars represent ± SEM (n = 5 slices). (A) At 26 weeks, the hippocampus of ApoE4xAPP mice was not sensitive to insulin stimulation. Insulin treatment resulted in a significant increase in P-Akt S473 (*p < 0.05, **p < 0.01) and P-Akt T308 (**p < 0.01) expression in ApoE3xAPP and APP mice. (B) At 52 weeks, both the hippocampus of ApoE4xAPP and APP mice were unresponsive to insulin stimulation. In ApoE3xAPP mice, insulin stimulation led to a marked increase in P-Akt S473 and P-Akt T308 expression (*p < 0.05, **p < 0.01). (C) At 78 weeks, insulin stimulation only increased P-Akt S473 and P-Akt T308 expression in ApoE3xAPP mice (*p < 0.05, **p < 0.01).