Table 1.
Independent variables and levels of experiment design
| Independent variables | Levels | ||||
|---|---|---|---|---|---|
| −1.682 | −1 | 0 | 1 | 1.682 | |
| X 1: amount of TET (mg) | 5 | 6.01 | 7.5 | 8.99 | 10 |
| X 2: amount of GMO (mg) | 200 | 260.81 | 350 | 439.19 | 500 |
| X 3: F127/GMO (%) | 9 | 10.22 | 12 | 13.78 | 15 |
A three-factor, five-level central composite design was generated to optimize the TET-LCNP preparation. The study design involved investigating the effects of three independent variables, including the amount of TET (X 1), the amount of GMO (X 2), and the F127/GMO ratio (X 3). The dependent variables were the entrapment efficiency (Y 1) and drug loading capacity (Y 2) of the TET-LCNPs