Figure 2.

Metabolic Symbiosis of Sunitinib-Treated Tumors

(A) Immunohistofluorescence staining of Ren28 and Ren96 RCC PDX tumors showing representative images of MCT1, GLUT1, MCT4, and CD31, counterstained with DAPI (20× magnifications).

(B) Quantification of expression of MCT1 and MCT4 in Ren28 and Ren96 as a percentage of positive area per field, from four animals per treatment group, four images each.

(C) Quantification of metabolic symbiosis pattern in Ren28- and Ren96-treated tumors as presence of MCT1/MCT4 mutually exclusive pattern (symbiosis pattern) versus homogeneous, co-staining (no symbiosis pattern).

(D) Representative images of pS6, MCT1, and MCT4 immunohistofluorescence staining in RCC PDX tumors.