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. 2016 May 17;5:639. doi: 10.1186/s40064-016-2293-3

Table 1.

Primers used in this study

Primer Sequence (5′–3′) Application Annealing temperature (°C)
ToF CTTGGGTATGCCGTCTAT Amplification of Tollip cDNA 59
ToR GTGCGAATCACCTCCTTA Amplification of Tollip cDNA 59
GSP3′-1 CAGCCTGTGGTCCTGATG 3′RACE specific out primer 55
GSP3′-2 CCTGCTGTTACATCACAGA 3′RACE specific inner primer 55
GSP5′-1 GGTTCCCTCTCGCAGGCTCTCT 5′RACE specific out primer 55
GSP5′-2 ACGCCCTCTCATCGAAGATTTC 5′RACE specific inner primer 55
U3′-1 TACCGTCGTTCCACTAGTGATTT 3′RACE universal out primer 55
U3′-2 CGCGGATCCTCCACTAGTGATTTCACTATAGG 3′RACE universal inner primer 55
U5′-1 CATGGCTACATGCTGACAGCCTA 5′RACE universal out primer 55
U5′-2 CGCGGATCCACAGCCTACTGATGATCAGTCGATG 5′RACE universal inner primer 55
S11F CCGTGGGTGACATCGTTACA Real-time PCR for S11 60
S11R TCAGGACATTGAACCTCACTGTCT Real-time PCR for S11 60
RT-TF CCGTCTCAGCATCACCGTCGTA Real-time PCR for Tollip 60
RT-TR TCGCTCCGTTGTGGGCAGTAG Real-time PCR for Tollip 60
IL-1βF GCTCGGCTTCATCTTGGAGAATGT Real-time PCR for IL-1β 60
IL-1βR GCAAGGTGAGGCTGGTCTTATTGT Real-time PCR for IL-1β 60
IL-6F TGAAGACAGTGATGGAGCAGCAGA Real-time PCR for IL-6 60
IL-6R CCTCACAGCAATGTGGCGAACA Real-time PCR for IL-6 60