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. 2016 May 18;6:26143. doi: 10.1038/srep26143

Figure 4. Aligned nanotopography resulted in decreased cytoskeletal stiffness.

Figure 4

(A) Electrospinning was used to fabricate randomly aligned and aligned nanofibers of 668 ± 98.6 nm and 621 ± 173 nm, respectively. Lack of surface topography is observed in smooth film. (B) AFM was used to determine cytoskeletal stiffness of tumor cells when cultured on differing topographic substrates. When cultured on aligned nanofibers, cytoskeletal stiffness significantly decreased (p < 0.05) compared to each other substrate for each cell type. (C) Representative fluorescent images of A172-GFP tumor cells on different substrates. N = 3, mean ± SEM.