FIG. 5.
ScNs showed HCN channels. (A) In voltage clamp, hyperpolarizing step from −60 to −90 mV resulted in the development of a slow inward current. This current was reversible blocked by cesium, identifying it as mediated by HCN channels (ie, Ih). (B) Voltage dependence of Ih was determined using 2-sec long steps from −60 to −110 mV. (C) The kinetics of Ih in ScNs was well fitted by two exponentials (red line), suggesting the presence of two components to the current, a smaller fast component and a larger slower component. Amp 1 and Amp 2 correspond to the amplitude of these two components and Tau1 and Tau 2 to their time constants. Color images available online at www.liebertpub.com/scd