Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 16;25(10):774–787. doi: 10.1089/scd.2016.0009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2016, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 2. — DC possess intracellular CTLA-4. Following CD14-selection, DC-differentiation, and CD11c enrichment, DC were analyzed for intracellular CTLA-4. CD11c⁺ DC were shown to possess intracellular CTLA-4 by (A) flow cytometry, (B) immunofluorescent confocal microscopy, and (C) RT-PCR. All methods revealed an increase in CTLA-4 quantity corresponding to DC maturation, and CTLA-4 siRNA successfully reduced CTLA-4 mRNA levels. (D) DC displayed a more global distribution of CTLA-4 than polarized, surface-bound CTLA-4 associated with T cells. (E) Tolerogenic DC differentiated with M-CSF and TGF-β possessed higher levels of intracellular CTLA-4 than conventional GM-CSF-/IL-4-differentiated DC. M-CSF, macrophage colony-stimulating factor.