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siRNA knockdown of CTLA-4 in CFSE-loaded DC diminishes uptake of CFSE-loaded EV by unlabeled recipient DC. (A, B) DC were loaded with 5 μM CFSE, treated with CTLA-4 or NT siRNA for 72 h, and matured. Culture supernatants were then collected and incubated with unlabeled DC for various lengths of time before flow cytometric analysis for levels of CFSE uptake and residual ability of CD80 (B7-1) to still be stained by specific antibodies.
