Table 1.

The Primers Used for Quantitative Polymerase Chain Reaction

Function	Gene	Primer	Sequence
Internal control	β-actin	5′-primer	GTGCGTGACATTAAGGAGAAGCTGTGC
		3′-primer	GTACTTGCGCTCAGGAGGAGCAATGAT
Transcription factors	EGR-1	5′-primer	AGTCTTTTCCTGACATCTCTCTGAA
		3′-primer	ACTAGGCCACTGACCAAGCTGAA
Angiogenic factors	TGF-β	5′-primer	AGAGCTCCGAGAAGCGGTACCTGAACCC
		3′-primer	GTTGATGTCCACTTGCAGTGTGTTATCC
	bFGF	5′-primer	AGAGCGACCCTCACATCAAGCTACAAC
		3′-primer	ATAGCTTTCTGCCCAGGTCCTGTTTTG
Adhesion molecules	Cyr61	5′-primer	TGGGTCTGTGACGAGGATAGTATCAAGG
		3′-primer	CTTGTAAAGGGTTGTATCGGATGCGAGG
	Col4	5′-primer	AGGGCCAGCCTGGCCTGCCAGGACTTCC
		3′-primer	TCACCCTTAGAGCCTGTGATTCCTGGAG
	Inav	5′-primer	ATACGACCCCAATGTTTACAGCATCAAG
		3′-primer	GCCAGTAAAATTGTATAAGGAGGACATG
Proinflammatory cytokine	IL-6	5′-primer	ACAAGAGTAACATGTGTGAAAGCAG
		3′-primer	TATACCTCAAACTCCAAAAGACCAG
Adipogenic markers	PPARγ2	5′-primer	GCCAAGGCTTCATGACAAGGGAGTTTC
		3′-primer	CACGTGTTCCGTGACAATCTGTCTGAG
	Adiponectin	5′-primer	CCTGGTGAGAAGGGTGAGAAAGGAGATCC
		3′-primer	TGTGATGTGGTAGGCAAAGTAGTACAGCC
Osteogenic markers	Runx2	5′-primer	CAGATGGGACTGTGGTTACTGTCATGG
		3′-primer	CCTAAATCACTGAGGCGGTCAGAGAAC
	ALP	5′-primer	ACGTGGCTAAGAATGTCATC
		3′-primer	CTGGTAGGCGATGTCCTTA
Mediators of insulin resistance	PTEN	5′-primer	TTG GCG GTG TCA TAA TGT CT
		3′-primer	GCA GAA AGA CTT GAA GGC GTA
	GGPS1	5′-primer	ACTGTTTGGATTAGCAGTAGGTCTC
		3′-primer	GGAGTGTAGATTAGCATAATCATCC