Figure 4. A quantitative analysis showed that the number of STAT3⁺ GFAP⁺ cells in Trpv1^+/+ mice was significantly increased in sensory CVOs and hypothalamic thermoregulatory subregions after the brain administration of 500 ng/kg RTX.

On the other hand, the brain administration of 500 ng/kg RTX did not elevate their number in Trpv1^−/− mice. The number of STAT3⁺ HuC/D⁺ neurons was also increased in Trpv1^+/+ mice, but was markedly lower than that in GFAP⁺ cells. Data were expressed as the mean (±s.e.m.) number of STAT3⁺ cells per 1 mm². *P < 0.05, **P < 0.01, ***P < 0.001 vs the vehicle in Trpv1^+/+ mice or ^###P < 0.001 between Trpv1^+/+ and Trpv1^−/− mice by ANOVA with Turkey’s post hoc tests or the Student’s t-test (n = 4).