Table 1. STAT3 activation in GFAP+ NSCs and astrocytes in the mouse brain by peripheral and icv administration of LPS and icv administration of RTX.
| Brain regions | Trpv1+/+ |
Trpv1−/− |
Trpv1+/+ |
Trpv1−/− |
Trpv1+/+ |
Trpv1−/− |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vehicle ip | LPS ip | Vehicle ip | LPS ip | Vehicle icv | LPS icv | Vehicle icv | LPS icv | Vehicle icv | RTX icv | Vehicle icv | RTX icv | |
| Forebrain | ||||||||||||
| Cerebrum | − | − | − | − | − | − | * | * | − | − | − | − |
| Cerebellum | − | − | − | − | − | − | * | * | − | − | − | − |
| Olfactory bulb | − | − | − | − | − | − | * | * | − | − | − | − |
| Limbic system | ||||||||||||
| Hippocampus | − | − | − | −− | − | − | * | * | − | − | − | − |
| Fimbria | − | −/+ | − | − | − | −/+ | * | * | − | −/+ | − | − |
| Ventral hippocampal commissure | − | ++ | − | −/+ | − | ++ | * | * | − | ++ | − | − |
| Hypothalamus | ||||||||||||
| Arcuate hypothalamic nucleus | − | + | − | − | − | + | * | * | − | + | − | − |
| Lateral septal nucleus | − | − | − | − | − | − | * | * | − | − | − | − |
| Median preoptic nucleus | − | + | − | + | − | +++ | − | − | − | +++ | − | − |
| Preoptic area | − | + | − | − | − | +++ | − | − | − | ++ | − | − |
| Periventricular hypothalamic nucleus | − | + | − | − | −/+ | + | * | * | −/+ | ++ | −/+ | −/+ |
| Paraventricular hypothalamic nucleus | − | + | − | − | − | + | * | * | − | ++ | − | − |
| Suprachiasmatic nucleus | − | − | − | − | − | − | * | * | − | −/+ | − | − |
| Supraoptic nucleus | − | + | − | + | − | ++ | * | * | − | −/+ | − | − |
| Supramammillary nucleus | − | − | − | − | − | − | * | * | − | − | − | − |
| Ventromedial hypothalamic nucleus | − | − | − | − | − | − | * | * | − | − | − | − |
| Circumventriculae organs | ||||||||||||
| Vascular organ of the lamina terminalis | − | ++ | − | + | − | +++ | − | − | − | +++ | − | − |
| Subfornical organ | − | +++ | − | + | − | +++ | − | − | − | +++ | − | − |
| Median eminence | −/+ | + | − | + | − | + | − | − | − | + | − | − |
| Area postrema | − | +++ | − | + | − | +++ | − | − | − | +++ | − | − |
| Brainstem | ||||||||||||
| Solitary nucleus | − | ++ | − | ++ | − | ++ | − | − | − | +++ | − | − |
| 10N | − | − | − | − | − | − | * | * | − | − | − | − |
| 12N | − | − | − | − | − | − | * | * | − | − | − | − |
| Other nonneuronal cells | ||||||||||||
| Meninges | − | ++ | − | ++ | −/+ | ++ | −/+ | −/+ | −/+ | + | −/+ | −/+ |
Relative values of nuclear STAT3 immunoreactivity are given as estimates of the density of nuclear STAT3 labeling. Nuclear STAT3 immunoreactivity was examined 2 hr after administration of LPS, RTX, or an equivalent volume of vehicle. A five point scale was used to rate nuclear translocation of STAT3: − none; −/+ none/week; + week; ++ moderate; +++ strong; *Not examined.