Table 1.

Summary of Common Additives in Decellularization Protocols and Their Effect on Assay Performance

Additive	Concentration	R2	Δ at 0.5% SDC
NaCl	1 M	0.98	+0.023
CaCl2	2 mM	0.94	+0.037
MgSO4	1.3 mM	0.98	+0.023
CaCl2 + MgSO4	2 mM +1.3 mM	0.98	−0.008
BSA	5 mg/mL	0.95	−0.016
	0.5 mg/mL	0.93	−0.002
	50 μg/mL	0.96	−0.006

R² is the value of the linear regression for the SDC standard curve with the given additive.

Δ at 0.5% SDC is the difference between the mean value of 0.5% SDC in deionized water and the 0.5% SDC with the additive.

BSA, bovine serum albumin; SDC, sodium deoxycholate.