Figure 1.

Diagram shows overall framework of a metabolic annotation strategy for linkage between sequence, structure, and function for annotating metabolic transporters in A. oryzae genome. In the 1st panel, Sets A and B indicate A. oryzae protein sequences searched against TCDB and TransportDB databases, respectively, under bidirectional best-hit analysis (BBH) and sensitivity analysis (SA). In the 4th panel, dash line implies the manual selection of a metabolic transporter from unambiguous function group as a representing case study of multilevel linkage annotation. SM and MD stand for SWISS-MODEL and molecular dynamics simulation, respectively.