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. 2016 May 10;2:16003. doi: 10.1038/celldisc.2016.3

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Copyright © 2016 SIBS, CAS

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Accumulation of PBR1 and Ycf1 is light-inducible during chloroplast development. (a) GUS-staining patterns of representative etiolated seedlings harboring Pro-PBR1::GUS constructs when exposed to light in 48 h. (b) Subcellular localization of PBR1-YFP (yellow fluorescent protein) during chloroplast maturation in protoplasts isolated from the cotyledons of representative etiolated seedlings harboring 35S::PBR1-YFP constructs during light illumination in 48 h. Bars=20 μm. (c) Immunodetection of accumulation of PBR1 and Ycf1 and abundance of thylakoid membrane proteins. The blots were probed with antibodies against the indicated proteins, respectively. Proteins were extracted from the wild-type etiolated seedlings during light illumination in 48 h. Protein samples were loaded on equal amounts of seedling fresh weight.