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. 2016 Apr 8;12(6):639–652. doi: 10.7150/ijbs.14025

Figure 1.

Figure 1

Characterization of human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSC-MSCs). Light microscopy images demonstrating that morphological changes that occur during hiPSCs differentiation into fibroblast-like cells. (A) Representative cell morphology of hiPSCs before differentiation. (B) Intermediate phase of the differentiating the hiPSCs into MSCs. (C) Typical fibroblast-like morphology of the cells. (D) Flow cytometric analysis of the surface markers in hiPSC-MSCs. Assessment of the tri-lineage differentiation capacity of iPSC-MSC-like cells. (E) Alizarin Red staining for bone matrix after 3 weeks in culture with osteogenic medium. (F) Toluidine blue staining for proteoglycans after 4 weeks in culture with chondrogenic medium. (G) Oil Red O staining for adipocytes after 2 weeks in culture with adipogenic medium. The qRT-PCR results for OCN and ALP (H), Sox9 and AGC (I), LPL and PPARγ (J) after 7 days in culture with osteo-, chondro-, and adipogenic mediun. Scale bar: 500 μm.