Figure 2.
Effects of DMOG on the proliferation, survival and angiogenic-related gene and protein expression of hiPSC-MSCs. (A) Effects of DMOG on the proliferation of hiPSC-MSCs was determined using CCK-8 after 24, 48, and 72 h. Effects of DMPG on the death ratio of hiPSC-MSCs in normal condition (B) or in serum deprivation condition (C) was determined using Live/Dead cell staining after 24, 48, and 72 h. (*p < 0.05 compared with the hiPSC-MSCs group, #p < 0.05 compared with the hBMSCs group). (D) Effects of DMOG on the angiogenic-related genes (HIF-1α, VEGF, SDF1, bFGF, and PLGF) or angiogenic-unrelated genes (Sox9) expression in hiPSC-MSCs after 3 and 7 days were detected using qRT-PCR (*p < 0.05). (E) Effects of DMOG on the protein expression of HIF-1α and VEGF in hiPSC-MSCs after 12, 24, and 72 h were detected using western blotting.
