Skip to main content
. 2016 Apr 8;12(6):639–652. doi: 10.7150/ijbs.14025

Figure 3.

Figure 3

Activation of the PI3k/Akt pathway in the angiogenic activity of the DMOG-hiPSC-MSCs group and the effects of conditioned medium (CM) on tube formation by HUVECs. (A) Expression profile of key proteins in the PI3k/Akt pathway in the hiPSC-MSCs and DMOG-treated hiPSC-MSCs groups at 12 h; the hiPSC-MSCs and DMOG-treated hiPSC-MSCs groups were cultured with or without LY294002. (B) Effects of DMOG on the receptors (VEGFR2, CXCR4, bFGFR, VEGFR1) expressions in HUVECs after 24 h were detected using qRT-PCR. (C) HUVECs tube formation was studied by growing cells in Matrigel in DMEM, hiPSC-MSCs CM, or DMOG-treated hiPSC-MSCs CM for 6 h, and quantitative analysis by (D) total branching points and (E) tube lengths. Scale bar: 250μm. (*p < 0.05)