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. 2016 Apr 8;12(6):639–652. doi: 10.7150/ijbs.14025

Figure 6.

Figure 6

New bone formation and mineralization was determined histomorphometrically through fluorochrome-labeling analysis and histological analysis of newly formed bone. (A) Column 1 (yellow), 2 (red) and 3 (green) represent staining with tetracycline after 2 weeks, alizarin red after 4 weeks, and calcein after 6 weeks, respectively. Column 4 represents the merged images of the three fluorochromes for the same group, and column 5 represents merged images from a plain microscope. (B) The graph shows the percentage of each fluorochrome area for the different groups. (C) The undecalcified craniums were sliced, and sections were stained with van Gieson's picrofuchsin. The new bone and β-TCP are shown in red and black, respectively. (D) There were significant differences in the new bone area between the β-TCP, hiPSC-MSCs, DMOG-treated hiPSC-MSCs and DMOG-hiPSC-MSCs+LY294002 groups (*p < 0.05 compared with the β-TCP group, #p < 0.05 compared with hiPSC-MSCs and DMOG-hiPSC-MSCs+LY294002groups, &p < 0.05 compared with hiPSC-MSCs group).