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. 2016 Apr 28;12(6):746–756. doi: 10.7150/ijbs.13988

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Effect of LCP on apoptosis in gastrointestinal cancer cells. The expression of apoptotic-related protein levels which including two anti-apoptotic proteins (Bcl-xL and Survivin) and two pro-apoptotic proteins (Caspase-3 and Caspase-8) were determined by Western blot in AGS cells (6A) and SW-480 cells (6B) according to LCP (10.0 mg/ml) or 5-FU (200 μM) treatment. 6C, Immunohistochemical staining of Bcl-xL and TUNEL analysis of apoptosis in AGS xenograft tissues. Control group (without LCP treatment): ① Bcl-xL protein by IHC; ② Apoptosis by TUNEL. LCP treated group (10.0 mg/ml LCP): ③ Bcl-xL protein by IHC; ④ apoptosis by TUNEL.