Fig. 2.

Phylogeny of Kinetoplastea based on a concatenation of 11 proteins. The tree was built using the C20 + Poisson model with Phylobayes 4.1. 1,000 bootstrap replicates were performed using the LG4X model IQTREE and mapped onto the nodes as percentages (left) alongside Bayesian posterior probabilities (right). Bootstrap values >50 % are shown, while only posterior probabilities >0.6 are shown. The topology of this tree, rooted with Diplonemida and Euglenida, is the same as in Additional file 3: Figure S1.1, which includes a more distantly related outgroup, Naegleria gruberi. In both trees, Prokinetoplastina (highlighted red) are the earliest branching kinetoplastid lineage. The bodonids branch as sister to the Trypanosomatida, while Leishmaniinae, Phytomonas and Strigomonadinae form a strongly supported, distinct group. Higher-level taxonomic classifications are indicated on the right for each group of organisms. The scale bar indicates the inferred number of substitutions per amino acid site