Table 1.

Presence/absence of enzymes involved in the biosynthesis of heme

		ALAS	gltX	GTR (HemA)	GSA-AT (HemL)	ALAD (HemB)	PBGD (HemC)	UROS (HemD)	UROD (HemE)	CPOX (HemF)	CPOX (HemN)	PPOX (HemY)	PPOX (HemG)	FeCH (HemH)
Naegleria	Naegleria gruberi		+		+						+			+
Euglenida	Eutreptiella gymnastica	+	+	+	+	+	+	+	+	+	+	+		+
Diplonemida	Diplonema papillatum *
Prokinetoplastina	putative Perkinsela sp. from Paramoeba atlantica		+
	putative Perkinsela sp. from Neoparamoeba aestuarina	+	+			+	+		+	+		+		+
	Perkinsela sp. from P. pemaquidensis CCAP1560	+	+			+	+		+	+		+		+
	Perkinsela sp. from P. invadens	+	+			+	+		+	+		+		+
Neobodonida	Neobodo designis		+
Eubodonida	Bodo saltans		+
Trypanosoma	Trypanosoma brucei TREU927		+
	Trypanosoma congolense IL3000		+
	Trypanosoma cruzi CL Brener Esmeraldo-like		+
	Trypanosoma cruzi CL Brener Non-Esmeraldo-like		+
	Trypanosoma cruzi marinkellei B7		+
	Trypanosoma evansi STIB805		+
	Trypanosoma grayi ANR4		+
	Trypanosoma rangeli SC58		+
	Trypanosoma vivax Y486		+
Phytomonas	Phytomonas Hart1		+											+
	Phytomonas EM1		+											+
Strigomonadinae	Strigomonas culicis		+							+			+	+
	Angomonas deanei		+							+			+	+
Leishmaniinae	Leptomonas pyrrhocoris		+							+			+	+
	Crithidia fasciculata CfCl		+					+		+			+	+
	Leishmania braziliensis MHOMBR75M2904		+					+		+			+	+
	Leishmania donovani BPK282A1		+					+		+			+	+
	Leishmania infantum JPCM5		+					+		+			+	+
	Leishmania major Friedlin		+					+		+			+	+
	Leishmania mexicana MHOMGT2001U1103		+					+		+			+	+
	Leishmania tarentolae ParrotTarII		+					+		+			+	+
Amoebozoa	Paramoeba pemaquidensis (host)	+	+			+		+	+		+	+
	Neoparamoeba aesturina (host)	+	+			+	+	+	+	+	+			+
	Paramoeba atlantica (host)	+	+			+		+	+	+	+	+

A ‘+’ indicates the presence of this enzyme. A ‘*’ indicates uncertainty associated with incomplete genomic or transcriptomic data. Blanks indicate protein absence or not detected