Fig. 5.

CRY and Pyx function in Johnston’s organ for GMF-modulated positive geotaxis. a, c, e Comparisons of the geotactic positioning of wild-type flies, the fly mutants for cry, pyx and pdf, and the mutant flies in which these genes were genetically restored using nan-GAL4 driver, a JO-specific GAL4 driver, respectively. Error bars: SEM. n.s.: not significant. **, P < 0.01; ***, P < 0.005; ****, P < 0.001 by Student’s t-test. b, d, f Comparisons of the geotactic positioning of wild-type flies, the fly mutants for cry, pyx and pdf, and the mutant flies in which these genes were genetically restored using iav-GAL4 driver, another JO-specific GAL4 driver, respectively. Error bars: SEM. n.s.: not significant. **, P < 0.01; ***, P < 0.005 by Student’s t-test. g, h Comparisons of the geotactic positioning of wild-type flies, control flies (GAL4 driver alone, UAS-pdf RNAi alone), and the flies with RNAi knockdown of pdf using cry-GAL4 or pyx-GAL4 driver, respectively. Error bars: SEM. n.s.: not significant. **, P < 0.01; ***, P < 0.005; ****, P < 0.001 by Student’s t-test. For all the data, n = 10 trials