Table 2.
Fermentation medium | Cultivation regimena | Amylase Activity | Total protein | Biomass (g/50 ml) |
---|---|---|---|---|
MoC | LSSF | 1.697 ± 0.27 | 521.87 ± 4.14 | 0.203 ± 0.013 |
SSF | 17.8 ± 2.33 | 7.928 ± 1.90 | − | |
NoC | LSSF | 0.49 ± 0.03 | 859.99 ± 1.21 | 0.094 ± 0.007 |
SSF | 1.17 ± 0.61 | 8.811 ± 1.97 | − | |
GnoC | LSSF | 0.354 ± 0.09 | 393.88 ± 1.58 | 0.243 ± 0.002 |
SSF | 2.11 ± 0.62 | 1.977 ± 0.78 | − | |
BGP | LSSF | 0.599 ± 0.34 | 870.54 ± 2.46 | 0.085 ± 0.002 |
SSF | 2.36 ± 0.14 | 6.539 ± 1.58 | − | |
GGP | LSSF | 0.697 ± 0.27 | 579.99 ± 2.22 | 0.157 ± 0.004 |
SSF | 7.44 ± 0.95 | 9.216 ± 2.25 | − | |
CVP | LSSF | 1.414 ± 0.234 | 1133.66 ± 7.89 | 0.2108 ± 0.006 |
SSF | 1.543 ± 0.387 | 12.89 ± 2.32 | − | |
WB | LSSF | 0.564 ± 0.07 | 582.77 ± 2.42 | 0.080 ± 0.001 |
SSF | 1.042 ± 0.25 | 3.133 ± 1.02 | − | |
PM | LSSF | 1.697 ± 0.22 | 246.19 ± 3.76 | 0.1412 ± 0.212 |
SSF | 19.19 ± 0.96 | 28.98 ± 5.66 | − | |
FM | LSSF | 0.981 ± 0.24 | 540.55 ± 2.35 | 0.032 ± 0.004 |
SSF | 7.44 ± 0.93 | 2.594 ± 0.88 | − | |
BR | LSSF | 0.599 ± 0.04 | 259 ± 0.073 | 0.1981 ± 0.0812 |
SSF | 2.36 ± 0.36 | 3.21 ± 0.921 | − | |
BP | LSSF | 0.101 ± 0.02 | 153.32 ± 4.26 | 0.017 ± 0.044 |
SSF | 1.94 ± 0.33 | 2.32 ± 0.79 | − | |
AP | LSSF | 0.152 ± 0.04 | 177.33 ± 1.211 | 0.2117 ± 0.0217 |
SSF | 1.74 ± 0.61 | 1.896 ± 0.511 | − | |
OP | LSSF | 0.126 ± 0.09 | 891.65 ± 2.66 | 0.031 ± 0.065 |
SSF | 0 ± 0.00 | 9.33 ± 2.09 | − | |
CONTROL* | LSSF | 0.668 ± 0.19 | 211.66 ± 2.88 | 0.109 ± 0.079 |
Control refers to the basal medium with 1% (w/v) starch as inducer of amylase.
The liquid static surface fermentation (LSSF) and solid state fermentation (SSF) experiments were performed for 96 h at 30°C. The data represent mean ± SD of replicates (n = 3). {Uml−1, μgml−1, and μmolml−1 are used for LSSF; Ugds−1, mggds−1, and μmolgds−1 are used for SSF}.
[Weight of biomass (W3) = weight of biomass and filter paper (W1) − only weight of filter paper (W2)].