Table 3.

Effect of amino acids, metal ions, vitamins and growth regulators on amylase production by A. terreus NCFT 4269.10^a.

Different sources	Amylase
	Biomass (g/50 ml)	Activity (U/ml)	Final pH*
AMINO ACIDS (5 mM/100 ml)
Alanine	0.10 ± 0.005c	1.27 ± 0.02c	4.6
Proline	0.09 ± 0.045d	2.53 ± 0.008ab	6.0
Valine	0.08 ± 0.004de	0 ± 0.00	4.3
Aspartic acid	0.09 ± 0.002d	0.8 ± 0.01d	4.2
Methionine	0.05 ± 0.025f	1.28 ± 0.02c	5.0
Glutamate	0.07 ± 0.009e	2.65 ± 0.17ab	4.5
L-lysine	0.10 ± 0.008c	0 ± 0.00	4.5
Cysteine	0.09 ± 0.044d	2.07 ± 0.04b	4.3
Histidine	0.09 ± 0.018d	2.49 ± 0.06b	4.1
Phenyl alanine	0.07 ± 0.024d	0.75 ± 0.01d	4.4
Isolucine	0.07 ± 0.003e	3.81 ± 0.009a	4.0
Threonine	0.06 ± 0.004ef	3.07 ± 0.02ab	5.0
Tryptophan	0.12 ± 0.016c	1.26 ± 0.003c	4.7
Agrinine	0.05 ± 0.001f	0 ± 0.00	3.8
Leucine	0.01 ± 0.001i	2.60 ± 0.09b	4.0
Glycine	0.04 ± 0.016g	2.04 ± 0.02b	5.4
Serine	0.07 ± 0.003e	0 ± 0.00	5.2
METAL IONS (1 mM)
Zn++	0.68 ± 0.013a	0.65 ± 0.062d	4.3
K+	0.72 ± 0.085a	0.92 ± 0.015cd	3.7
Ag++	0.05 ± 0.014f	0.81 ± 0.051cd	4.4
Fe++	0.58 ± 0.004b	0.42 ± 0.006e	3.4
Mg++	0.68 ± 0.141ab	2.33 ± 0.145b	4.4
Cu++	0.02 ± 0.001h	0.55 ± 0.004e	3.7
Mn+	0.01 ± 0.000i	0.93 ± 0.022d	8.6
Ca+	0.70 ± 0.170a	1.94 ± 0.041c	4.2
Hg++	0.02 ± 0.003h	0.52 ± 0.022e	4.0
EDTA	0.03 ± 0.000g	0.76 ± 0.004d	5.4
VITAMINS (mg/100 ml)
Vitamin C
10	0.38 ± 0.10e	19.7 ± 0.7e	6.9
20	0.12 ± 0.00g	4.8 ± 1.3f	6.4
30	0.73 ± 0.09b	54.1 ± 4.9c	6.1
40	0.43 ± 0.01de	25.1 ± 0.6d	6.4
50	0.2 ± 0.00f	4.9 ± 1.7f	6.5
Riboflavin
10	0.57 ± 0.00cd	33.1 ± 3.7d	6.2
20	0.14 ± 0.00g	2.1 ± 3.0g	6.6
30	0.66 ± 0.08cd	58.8 ± 2.2c	6.6
40	0.73 ± 0.06b	92.3 ± 1.3b	6.7
50	0.09 ± 0.00h	2.2 ± 0.6g	6.2
Folic acid
10	0.08 ± 0.00h	0.3 ± 0.4i	5.2
20	0.07 ± 0.00h	0.6 ± 0.4h	5.1
30	0.04 ± 0.00i	0 ± 0.0	5.4
40	0.11 ± 0.00g	6.7 ± 0.4f	5.3
50	0.43 ± 0.07d	22.1 ± 1.9de	5.5
Vitamin E
10	0.08 ± 0.00gh	0.9 ± 0.4h	6.3
20	0.06 ± 0.00hi	0.3 ± 0.4i	6.2
30	0.85 ± 0.06a	101.2 ± 10.7a	5.7
40	0.45 ± 0.00d	39.5 ± 0.2d	6.3
50	0.43 ± 0.01d	41.2 ± 22.3cd	6.4
GROWTH REGULATORS (0.0025 mg/g, w/w)
Gibberellic acid	0.12 ± 0.02a	2.03 ± 0.07a	5.2
Kinetin	0.05 ± 0.02c	0.24 ± 0.00cd	4.7
6-Benzylaminopurine	0.07 ± 0.02bc	0.60 ± 0.03b	4.9
2,4-Dichlorophenoxyacetic acid	0.05 ± 0.01c	0.47 ± 0.0d	5.3

^aThe liquid static surface culture experiments were performed for 4 days at 30°C for all the cases using PM as the substrate.

pH

^*, Initial pH was adjusted 7.0 required for the biosynthesis of amylase.

*Control refers to the PM added fermentation broth medium (10% w/v) devoid of amino acids, metal ions, vitamins and growth regulators. The data represent mean ± SD of replicates (n = 3). Mean values within a column with different letters are significantly different at p ≤ 0.05.