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Purification summary of isolated amylase.
| Steps | Volume | Activity (Uml−1) | Total enzyme activity (U) | Total protein (mg) | Specific activity (Umg−1) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|---|
| Crude | 540 | 1.91 | 1031 | 155.99 | 6.6089 | 1.0 | 100 |
| Ammonium sulphate precipitation | 15 | 25.4 | 381 | 25.0 | 15.24 | 2.305 | 36.95 |
| Sephadex G-100 | 5 | 56.4 | 282 | 4.6 | 61.304 | 9.276 | 27.35 |
