Figure 2.
Photomicrographs of pro-NKB immunohistochemistry from a representative Blank-SAP and NK3-SAP rat. A, The Blank-SAP control shows intense staining of NKB neuronal elements in the arcuate nucleus. There is jet-black staining of scattered cell bodies with a dense plexus of NKB axons. B, In contrast, rats with accurate targeting of NK3-SAP injections did not show the intense jet-black staining of neurons but only a faint cellular blush consistent with background staining. In addition, the dense plexus of NKB axons within the arcuate neuropil was absent. 3V, third ventricle; ARC, arcuate nucleus. Scale bar, 100 μm (applies to A and B).