Age-dependent changes in pancreatic RNA markers of endocrine function. A–I, Lysates of CF and non-CF pancreata were used for simultaneous detection of target RNA transcripts normalized to the housekeeping gene PPIB. The ratios of RNA transcript abundance for the indicated phases (ie, age groups) are shown for (A) insulin to PPIB, (B) glucagon to PPIB, (C) somatostatin to PPIB, (D) PP to PPIB, (E) Glut2 to PPIB, (F) PDX1 to PPIB, (G) PPARγ to PPIB, (H) adiponectin to PPIB, and (I) Sox9 to PPIB. Results in A–I show the mean ± SEM with significant differences by one-way ANOVA and Bonferroni post hoc test for the marked genotypic comparisons or within a genotype when marked by brackets: *, P < .05; **, P < .01; ***, P < .001. The number of independent animals in various groups for all graphs is marked in brackets above the bar graph in A. Phase NB, newborn; phase I, 9–19 days; phase II, 26–53 days; phase III, 68–120 days; phase IV, 4–12 months.