Skip to main content
. 2016 Mar 16;157(5):2052–2066. doi: 10.1210/en.2015-1958

Figure 2.

Figure 2.

HO-1, nNOS, and VP mRNA expression in PVN MNCs in HF rats. A, Bar graphs summarizing the qRT-PCR data analysis of HO-1, nNOS, and VP mRNA expression in the PVN of sham (open bar, n = 8) and HF (close bar, n = 8) rats. B1, Representative high magnification image showing an identified PVN eGFP-VP neuron under an infrared differential interference contrast (IR-DIC) (left) and DIC combined with fluorescence illumination (right). Note the presence of the patch pipette in the images showing that identified VP neurons were utilized to perform single-cell RT-PCR and patch-clamp recordings protocols. Scale bar, 10 μm. B2, Summary of single-cell RT-PCR data analysis showing the relative mRNA expression of HO-1, nNOS, and VP in identified PVN VP neurons of sham (open bar, n = 12) and HF (close bar, n = 14) rats. Data are reported as the mean ± SEM. Student's unpaired t test. *, P < .05 vs sham.