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Circulating IGF-1 and IGF-1 mRNA quantification. A, Circulating levels of IGF-1 at 4.5, 8.5, and 12.5 months of age in iC-GHRKO (n = 8) and control (n = 24) littermates. B, RTqPCR analysis of IGF-1 in heart and liver of iC-GHRKO (n = 5) and control (n = 15) littermates at 12.5 months of age. *, Significant difference between genotypes (P < .05).
