Figure 1.

Time course demonstrating TSH-mediated up-regulation of mRNA and protein expression of IL-11, OPN, and ALPL. U2OS-TSHR cells were treated with 100-mU/mL bTSH in EMEM supplemented with 0.1% BSA. Cell lysates were analyzed by RT-qPCR. ALPL was detected by Western blotting using tissue-nonspecific anti-ALPL antibodies. IL-11 and OPN secretion were measured by ELISAs in cell culture medium. The data are from 3 independent experiments with duplicate samples and are presented as the mean ± SEM.