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M22 and thyrostimulin exert effects similar to TSH on up-regulation of IL-11, OPN, and ALPL. U2OS-TSHR cells were treated with 300-ng/mL M22 and 200-ng/mL thyrostimulin in EMEM supplemented with 0.1% BSA for 5 days. Cell lysates were analyzed by RT-qPCR. The data are from 3 independent experiments with duplicate samples and are presented as the mean ± SEM.
