Figure 2.

Photomicrographs of human midbrain cell cultures.

The human midbrain (ReNcell-VM) cell line was grown for 1 week in control medium (A) or exposed to endothelial progenitor cells (EPC)-derived conditioned medium (B). The cultures were then immunocytologically stained for the neuronal marker beta-III-tubulin (in green) and the nuclear marker dapi (in blue). Note the higher fiber network of neuronal cells in the EPC-derived conditioned medium treated cultures. Moreover, EPC-derived conditioned medium administration induced a dopaminergic phenotype as demonstrated by the presence of some tyrosine hydroxylase (TH; in red) positive neurons (inserts, boxed area in B). Scale bars: 50 μm.