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PCR analysis of inflammatory cytokine mRNA levels in BPH-1 cells infected with T. vaginalis (A-D: CXCL8, CCL2, IL-1β, and IL-6). BPH-1 cells (2×105) were incubated with increasing numbers of live trichomonads (BPH-1: T. vaginalis=1:1, 1:5, and 1:10), for 45 min (CXCL8, CCL2, and IL-6) or 30 min (IL-1β). C, BPH-1 cells alone.
