Figure 1. Fluorescence microscopy of co-cultured trypanosomes expressing DsRED or GFP.

A: Double-positive trypanosome after 24 hours co-culture (WT). B: Interacting double-positive trypanosome pair found after 24 hours co-culture (Δproc). C: Double-positive trypanosomes connected at their anterior ends (Δproc). The scale bar indicates 10μm. DsRed tends to accumulate in the nuclei of cells that synthesise it, probably because of its propensity to form tetramers.