Figure 1.

Effect of siRNA knockdown of TLS polymerases on the extent of replicative bypass of dG-N²-MC (in 5′-CTAGTCG*TATCC-3′) and dG-N²-2,7-DAM (in 5′-CTAGTGG*TATCC-3′). Percent TLS in various polymerase knockdowns was measured using an internal control of unmodified plasmid in which a different 12-mer was ligated. The data represent the mean and standard deviation of results from two independent experiments. HEK 293T cells were treated with negative control (NC) siRNA (WT), whereas the other single, double, or triple polymerase knockdowns are indicated above the bar. TLS result from each knockdown experiment was considered statistically significant (p < 0.02) (except in pol η-knockdown cells) compared to that from HEK 293T cells treated with NC siRNA (WT). The p value of %TLS for each knockdown was calculated using a two-tailed, unpaired Student’s t test.