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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: J Clin Neurosci. 2009 May 26;16(8):1061–1063. doi: 10.1016/j.jocn.2008.12.011

Fig. 1.

Fig. 1

Serotonin receptor 2C (HTR2C) gene amplified by real-time polymerase chain reaction (PCR) from knock-out mice (lane 2) and controls (lane 3). Lane 1 shows molecular weight markers. Contemporary amplification of the housekeeping GAPDH gene was also performed (data not shown). Amplification kinetics, which showed an increase in expression in the hypoxanthine-guanine phosphoribosyl-transferase gene (HPRT) knock-out mice brains compared to controls, were obtained by analysing PCR samples at different steps (25, 27, 29, 30, 33 cycles).